Lateral flow assay production

Empower your lateral flow assays with cutting-edge precision and optimization.

As the gold standard for rapid visual detection tests in point-of-care diagnostics, lateral flow assays have evolved to meet increasing complexities. Integration of nanotechnologies and the need to determine multiple analytes within a single test demand advanced and precise production techniques. Elevate your lateral flow assays with our optimized solutions, ensuring accuracy, efficiency, and adaptability in the ever-evolving landscape of diagnostic technologies.

Non-contact dot or line printing

Leverage piezoelectric drop-on-demand dispensing for nanoliter droplet volumes, offering unparalleled flexibility during the developmental stage and robust performance for mass production.

Experience complete freedom in designing array layouts during development, while benefiting from a reliable and efficient dispensing mechanism for large-scale production. Our piezoelectric technology ensures precision, versatility, and consistency throughout the entire process, enhancing both innovation and scalability in your projects.

Adjust the line width

Tailor your dispensed lines with precision by setting droplet volumes using the SMARTDROP® System.

Achieve desired line thickness effectively:

  • Customizable Droplet Volumes: Set precise droplet volumes with SMARTDROP® to control line thickness.
  • Fine-Tuned Precision: Smaller single droplet volumes result in reduced line thickness, providing the exact specifications you need for your substrate.

Experience enhanced control and accuracy in your dispensing processes, ensuring that your lines meet the desired thickness with precision and efficiency.

Relevant literature

Whitepaper on the development and production of Lateral Flow Assay Test Stripes (LFA)

Lateral flow assays (LFAs) are widely used for rapid diagnostic testing and initial screening. A one-step and low-cost analysis of an analyte in a sample solution, such as pathogens, biomarkers and chemical contaminants, makes it a powerful point-of-care device without the need of trained personnel. Read how to optimize, develop and to produce LFA applying BioFluidix technology.

Iridium oxide (IV) nanoparticle-based lateral flow immunoassay | 2019

Nanomaterials have been widely reported in lateral flow biosensors, offering new sensing strategies based on optical or electrical detection techniques. Looking for other advantageous nanomaterials, we propose for the first time the use of iridium oxide (IV) nanoparticles in lateral flow assays for the detection of human immunoglobulin as a model protein.

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Signal enhancement on gold nanoparticle-based lateral flow tests using cellulose nanofibers | 2019

Lateral flow paper-based biosensors merge as powerful tools in point-of-care diagnostics since they are cheap, portable, robust, selective, fast and easy to use. However, the sensitivity of this type of biosensors is not always as high as required, often not permitting a clear quantification. To improve the colorimetric response of standard lateral flow strips (LFs), we have applied a new enhancement strategy that increases the sensitivity of LFs based on the use of cellulose nanofibers (CNF). CNF penetrate inside the pores of LFs nitrocellulose paper, compacting the pore size only in the test line, particularly near the surface of the strip. This modification retains the bioreceptors (antibodies) close to the surface of the strips, and thus further increasing the density of selectively attached gold nanoparticles (AuNPs) in the top part of the membrane, in the test line area, only when the sample is positive.

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Flow control for lateral flow strips with centrifugal microfluidics | 2019

Lateral flow strips (LFSs) are widely used for clinical diagnostics. The restricted flow control of the current designs is one challenge to the development of quantitative and highly sensitive LFSs. Here, we present a flow control for LFSs using centrifugal microfluidics. In contrast to previously presented implementations of lateral flow membranes into centrifugal microfluidic cartridges, we direct the flow radially outwards through the membrane. We control the flow using only the centrifugal force, thus it is independent of membrane wetting properties and permeability. The flow rate can be decreased and increased, enabling control of incubation times for a wide variety of samples.

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Uranium (VI) detection in groundwater using a gold nanoparticle/paper-based lateral flow device | 2018

The contamination in groundwater due to the presence of uranium is nowadays a subject of concern due to the severe health problems associated with renal failure, genotoxicity and cancer. [...] For the first time, we propose a portable, fast, inexpensive and sensitive paper-based biosensor able to detect in situ U(VI) in water samples: U(VI) selective gold nanoparticle-based lateral flow strips. Antibody-coated gold nanoparticles are used as labels in the proposed lateral flow system because of their biocompatibility; in addition, these nanoparticles provide high sensitivity due to their intense plasmonic effect.

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Photoluminescent lateral flow based on non-radiative energy transfer for protein detection in human serum | 2018

The assay is intended for the detection of a model protein in human serum, that is, human immunoglobulin G, with the aim to demonstrate a virtually universal protein detection platform. Once the sample is added in the strip, the analyte is selectively captured by antibody-decorated silica beads (Ab-SiO2) onto the conjugate pad and the sample flows by capillarity throughout the strip until reaching the test line, where a sandwich-like immunocomplex takes place due to the presence of antibody-functionalized QDs (Ab-QDs) onto the test line.

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Wax gates in laminated microfluidic paper-based immunosensors

A hydrophobic wax barrier (so-called a “wax gate”) combined with the use of surfactants was developed as a valving mechanism in paper-based microfluidic systems to enable the control of delays in reagent addition in the device. This mechanism allowed the delay of reagent delivery and assisted multistep analysis on microfluidic paper-based analytical device (μPADs).

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